RosettaCommons Blog

We are pleased to announce registration has opened for our 2024 Rosetta Workshop to be held July 22-24 in person at Vanderbilt University in Nashville, TN. The Vanderbilt Rosetta Workshop is designed to be an introduction to the Rosetta macromolecular modeling suite, focusing on a practical introduction to common Rosetta protocols. Registration for the workshop is on a first-come-first-served basis and is limited to 15 participants. Please register as soon as possible since spots fill quickly.   Participants are responsible for their own travel and accommodation arrangements. We have a block of rooms reserved at a discounted rate, and this information will be shared once registration is completed. For more information, and to register for the workshop, please visit  https://meilerlab.org/rosetta- workshop-summer-2024/ Questions can be directed at Rocco Moretti.

The Winter RosettaCon organizers want to thank Liberum Bio for sponsoring the experimental validation of the designs that they carried out in 8 days starting from digital sequences! Liberum Bio produces proteins at high yields in cell-free systems at a fraction of the usual cost and time via highly engineered strains and state-of-the art reaction mixtures. At Winter RosettaCon 2024 in Boston, the Rosetta Commons in partnership with Liberum Biotech hosted the inaugural Winter Protein Design Games , focusing on the design of Tobacco Etch Virus (TEV) protease variants. TEV protease is commonly used as a tool for sequence-specific protein cleavage, facilitating the purification of proteins without affinity tags. The competition challenged cross-institutional participating teams to engineer TEV proteases that exhibited enhanced substrate cleavage efficiency over the wild-type enzyme.  The competition was straightforward: design TEV protease variants that outperform the wild type in cl...

~  contributed by Dr. Jared Adolf-Bryfogle and Frances Chu Integrated Logic Selection  By  Dr. Jared Adolf-Bryfogle, Principal Scientist, Protein Design Lab, Institute for Protein  Innovation RosettaScripts has progressed to become one of the main interfaces to the Rosetta Software Suite.  Within RosettaScripts, there are a number of different sections that enable customized protocols.  Two of the newest components are ResidueSelectors and SimpleMetrics .  ResidueSelectors allow one to select a group of residues based on some criteria, while SimpleMetrics allow one to output a variety of metrics for each molecule under consideration, with many of them being per-residue metrics. I introduced SimpleMetrics to make benchmarking and analysis tasks easier.  For simple tasks, a single ResidueSelector is usually sufficient, but for more real-world applications, one usually would need to define large logic strings to get at the residues of interest....